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Promega S1 Nuclease
Description
- Endonuclease Specific for Single-Stranded DNA and RNA
- Enzyme yields 5′-phosphoryl-terminated products
- Double-stranded nucleic acids (DNA:DNA, DNA:RNA or RNA:RNA) resist degradation except with extremely high concentrations of enzyme
- Supplied at a concentration of 20-100u/μL
Specifications
Specifications
| Concentration | 20 to 100 U/μL |
| Color | Colorless |
| Components | 10X Reaction Buffer: Sodium Acetate 0.5M (pH 4.5 at 25°C), NaCl 2.8M, ZnSO4 45mM |
| pH | 7.5 |
| For Use With (Application) | Removal of protruding single-stranded termini in double-stranded DNA, selective cleavage of single-stranded DNA, mapping RNA transcripts |
| Storage Buffer | 20mM Tris HCl (pH 7.5 at 25°C), 0.1mM ZnCl2, 50mM NaCl, 50% Glycerol |
| Content And Storage | -30°C to -10°C |
| Source | Fungal a-amylase |
| Quantity | 10,000 U |
| Product Type | S1 Nuclease |
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