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Promega™ psiCHECK™ Vectors


Provide a quantitative and rapid approach for initial optimization of RNA interference (RNAi)

Manufacturer: promega™  C8021

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Catalog No. PR-C8021

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Description & Specifications

Specifications

For Use With (Application) Provide a quantitative and rapid approach for initial optimization of RNA interference (RNAi), monitors changes in expression of target gene
Reporter Gene Renilla Luciferase, Firefly Luciferase
Quantity 20μg
Vector psiCHECK-2 vector
Storage Requirements -20°C

Use Renilla luciferase as the primary reporter gene. Enable monitoring of changes in expression of a target gene fused to the reporter gene based on decreases in Renilla activity.

  • Protocols allow measurement in live cells or crude cell lysates
  • Quantitation can be performed with any luminometer; requires no expensive equipment
  • Provide higher sensitivity than GFP or flag-tag assays
  • No need to wait for phenotypic changes
  • No requirement for transfection normalization when using the psiCHECK-2 Vector