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Promega MagneHis™ Protein Purification System 
The MagneHis Protein Purification System provides a magnetic-based, rapid method for the purification of polyhistidine- or HQ-tagged, expressed proteins.
Supplier: Promega V8550
Includes: MagneHis binding/wash buffer, 50mL; FastBreak™ cell lysis reagent, 15mL; DNase I, lyophilized, 1 vial; MagneHis elution buffer, 10mL; MagneHis Ni-particles, 2mL
Description
- Magnetic Purification of Polyhistidine- or HQ-tagged Proteins from E. coli or Mammalian Cell Lysates
- No centrifugation or vacuum required after cell lysis
- Particles are compatible with a variety of common buffers
- Binding capacity up to 1mg of polyhistidine-tagged protein per 1mL of particles
- U.S. Pat. Nos. 7,112,552; 7,304,015; 7,304,016; 7,354,750; 6,174,704; and 7,319,021 and other patents and patents pending.
- Certain applications of this product are covered by patents issued and applicable in certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.
Specifications
| 50mL MagneHis binding/Wash Buffer, 15mL FastBreak™ cell lysis reagent, DNase I, 1 vial lyophilized, 10mL MagneHis Elution Buffer, 2mL MagneHis Ni-particles | |
| 325 Reactions | |
| Magnetic Bead Protein Purification System |
| 2°C to 10°C | |
| Rapid and reliable purification of Polyhistidine-tagged Proteins from 1mL to 1L of bacterial culture |