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Promega™ GoTaq™ DNA Polymerase

Provide robust amplification and streamlined analysis of PCR products, flexibility to optimize Mg2+ concentration, save time during reaction setup, and PCR product analysis

$36.00 - $710.00

Specifications

Concentration 5U/μL
For Use With (Application) PCR, two-step RT-PCR and T-vector cloning
Purity >90% Pure
Storage Requirements -20°C
View More Specs
Includes: 

  • GoTaq DNA Polymerase; 5X Colorless GoTaq Reaction Buffer (Used when direct fluorescence or absorbance readings are required without prior purification of the DNA from the amplification reaction); 5X Green GoTaq Reaction Buffer (Contains two dyes that separate during electrophoresis to facilitate direct-to-gel analysis of PCR products). Both buffers are supplied at pH 8.5 and contain MgCl2 at a concentration of 7.5mM, giving a final concentration of 1.5mM in a 1X reaction
  • GoTaq Green Master Mix or GoTaq Colorless Master Mix, nuclease-free water

Products
Catalog Number Mfr. No. Includes Quantity Price Quantity    

PR-M3001

 
promega™
M3001
GoTaq™ DNA Polymerase, 1mL 5X green GoTaq™ reaction Buffer and 1mL 5X colorless GoTaq™ reaction Buffer, MgCl2 at a concentration of 7.5mM, nuclease free water 100U Each for $36.00

PR-M3008

 
promega™
M3008
GoTaq™ DNA Polymerase, 4 x 5mL 5X green GoTaq™ reaction Buffer and 5X colorless GoTaq™ reaction Buffer, MgCl2 at a concentration of 7.5mM, nuclease free water 2500U Each for $710.00

PR-M3005

 
promega™
M3005
GoTaq™ DNA Polymerase, 4 x 1mL 5X Green GoTaq™ reaction Buffer and 4 x 1mL 5X colorless GoTaq™ reaction Buffer, MgCl2 at a concentration of 7.5mM, nuclease free water 500U Each for $161.00
Description & Specifications

Specifications

Concentration 5U/μL
For Use With (Application) PCR, two-step RT-PCR and T-vector cloning
Purity >90% Pure
Storage Requirements -20°C

  • Offer robust amplification equal to—and in some cases superior to—conventional Taq DNA polymerase
  • Suitable for PCR, two-step RT-PCR, and T-vector cloning
  • Samples can be loaded directly onto a gel after amplification
  • Compatible with PCR enhancers such as betaine and DMSO
  • Premixed, ready-to-use solutions contain GoTaq DNA polymerase, dNTPs, MgCl2, and reaction buffers in a single tube
  • Utilize an optimized enzyme and buffer formulation that offers amplification typically better than standard Taq DNA polymerases
  • Can be directly substituted into existing PCR protocols without the need to change cycling parameters
  • GoTaq Green Master Mix contains a blue dye and a yellow dye that allow the monitoring of progress during electrophoresis
  • Utilize a separate MgCl2 solution to allow for optimization of magnesium concentration
  • Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74°C. The reaction conditions are 50mM Tris-HCl (pH 9.0 at 25°C), 50mM NaCl, 5mM MgCl2, 200μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10μg activated calf thymus DNA and 0.1mg/mL BSA in a final volume of 50μL

The purchase of this product conveys to the buyer the limited, nonexclusive, nontransferable right (without the right to resell, repackage, or further sublicense) under U.S. Published Patent Appln. 20020150900 and U.S. Pat. Nos. 5,432,272, 6,617,106 and 6,140,496 to use the product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include or carry any right or license to sell this product. For information on purchasing a license for other uses, please contact Promega Corporation, Licensing, 2800 Woods Hollow Road, Madison, WI 53711, or EraGen Biosciences, Corporate Licensing, 918 Deming Way, Suite 201, Madison, WI 53717. Phone (608) 662-9000; Fax (608) 662-9003.
This product is designed and sold for use in the multiplex PCR process covered by U.S. Pat. No. 5,582,989 and Canadian Pat. No. 1,339,731. A limited license has been granted under the patent to use only this amount of the product to practice the multiplex PCR process and is conveyed to the purchaser by the purchase of this product.