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Promega Arg-C Sequencing Grade Modifying Enzyme

Catalog No. PRV1881
Encompass
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10 μg
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Catalog No. Quantity
PRV1881 10 μg
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Catalog No. PRV1881 Supplier Promega Supplier No. V1881
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Description

Arg-C (clostripain) is an endopeptidase that cleaves at the C-terminus of arginine residues, including the sites next to proline. Cleavage also will occur at lysine residues. Arg-C activity is optimal in the pH range of 7.6-7.9.

  • Sequencing-Grade Protease: for use Alone or with other Proteases for Mass Spectrometry Analysis
  • Cleaves at the C-terminus of arginine residues, including sites next to proline
  • Also cleaves at lysine residues
  • Activity is optimal in the pH range of 7.6-7.9

Specifications

Color Colorless
pH 7.6 to 7.9
For Use With (Application) Peptide mapping, Protein identification, characterization of post-translational modifications
Storage Buffer 50mM Tris HCl (pH 7.6 to 7.9), 5mM CaCl2, 2mM EDTA
Content And Storage 2°C to 10°C
Source Clostridium histolyticum
Quantity 10 μg
Product Type Arg-C (clostripain)
Molecular Weight (g/mol) 45 and 12
Inhibitors Oxidizing agents and Sulfhydryl reactants, also Co2+, Cu2+, Cd2+ and heavy Metal Ions, Citrate, Borate and Tris anions partially inhibit activity, TLCK (Tosyllysine Chloromethyl Ketone) reacts with active site (1,5,6)
Form Lyophilized Powder
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