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Applied Biosystems™ ProFlex™ PCR System, 3 x 32-well

Catalog No. A30754
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3 years of total service coverage
Limited Warranty
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A30754 3 years of total service coverage
4484073 Limited Warranty
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Catalog No. A30754 Supplier Applied Biosystems™ Supplier No. A30754

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

ProFlex™ PCR Systems are the latest of Applied Biosystems™ thermal cyclers, providing the reliability and performance you've come to expect from Applied Biosystems instruments. By combining an extended warranty with your instrument purchase, you'll extend the life of your investment and receive the support you need to keep your instrument running at peak performance.

The ProFlex 3 x 32-well PCR system is the latest in our line of Applied Biosystems thermal cyclers. The ProFlex system combines the reliability and performance you've come to expect from Applied Biosystems instruments with the flexible configuration and control features that adapt to your research needs.

The ProFlex 3 x 32-well PCR System allows you to:

  • Run three experiments at once with the 3 x 32-well, 0.2-mL block
  • Interchange the 3 x 32-well block with another block type if throughput needs change
  • Access the system remotely (and conveniently) through a mobile app
  • Program the instrument in seconds with a simple-to-use touchscreen interface
  • Simulate old instrument with Thermal Simulation modes
  • Optimize PCR quickly and efficiently with better-than-gradient VeriFlex blocks

Independent control

Share your instrument with two other people or run three experiments at once with three independently controlled 32-well modules.

Interchangeable blocks

If your research requirements change in the future, or if you share the system with someone who requires a different format, simply remove the 3 x 32-well block from the top of the instrument and exchange it with another block type. This step takes typically less than 15 seconds and requires no tools or kits.

Mobile app connectivity

Have you ever set up all of your PCR reactions, only to find out that the thermal cycler is already in use? Check its availability with a mobile app before you get to the lab. Also download experimental report files, receive alerts, and more.

Simulation modes

The ProFlex PCR System is equipped with simulation modes that mimic your old thermal cycler's ramp rate. Simulation modes are available for MJ Research PTC 200, Bio-Rad™ C1000, Bio-Rad MyCycler™, Eppendorf™ Mastercycler™, Applied Biosystems 9700, Applied Biosystems 9600, and Applied Biosystems Veriti systems.

VeriFlex blocks: enhanced PCR functionality

VeriFlex blocks are controlled by multiple Peltier elements (two separate Peltier elements per 32-well module). This gives you the ultimate control of your block for precise, 'better-than-gradient' optimization experiments and for running multiple reaction conditions at once for precise incubations and other non-PCR workflows.

8.4-inch touchscreen control

The simple and intuitive touchscreen interface allows you to program with ease and limits the amount of time spent training yourself and others how to use the system.

Specifications

Block Format Interchangeable
Thermal Accuracy ±0.25°C (35°C to 99.9°C)
High-throughput Compatibility High-throughput Compatible
Thermal Uniformity <0.5°C (20 sec. after reaching 95°C)
Reaction Speed Fast, Standard
Dimensions 22 x 13 x 10.6 in. (56.5 x 33 x 27 cm)
Capacity 3 x 32-well, 0.2 mL Tubes
Voltage 100/240 V
Product Line ProFlex
Display Type 8.4 in. Color TFT LCD Touchscreen
Product Type Thermal Cycler
Warranty 3 years of total service coverage
Thermal Range 0°C to 100°C
For Use With (Application) PCR
Programs Auto re-start (after Power Outages), Program Overwrite Protection
User Interface USB, WiFi, Cloud, Ethernet
Max. Ramp Rate 6°C/sec. (Block), 4.4°C/sec. (Sample)
Quantity System + 1 yr ABRC ext warranty
Memory USB and On-board
Weight 41 lb. (18.8 kg)
Block Configurations 3 x 32-well, 2-Zone VeriFlex Blocks
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What is the default admin password for a new ProFlex 3x32-well PCR System (Cat. No. 4484073)?

The default admin password for a new ProFlex 3x32-well PCR System is "passwordXXXX" with XXXX being the last four digits of the serial number.

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

For Research Use Only. Not for use in diagnostic procedures.