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Invitrogen™ ProcartaPlex™ Cell Lysis Buffer

Catalog No. 501125868
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20 mL
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Catalog No. Quantity
501125868 20 mL
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Catalog No. 501125868 Supplier Invitrogen™ Supplier No. EPX99999000
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Description

Used to lyse cells under nondenaturing conditions. Invitrogen™ ProcartaPlex™ Cell Lysis Buffer is available in 20mL units that should be stored chilled (for continual use) or frozen (for longer periods of time.) Additional protease inhibitors can be added without any difficulties.

For lysis of adherent cells, 400μL of cell lysis buffer is used for a 10cm dish. For non-adherent cells, 400μL of buffer is used per 107 cells, once they have been washed in PBS and pelleted. Buffer should be chilled and PMSF should be added to the buffer immediately prior to use.

Buffer composition:

  • 20mM Tris-HCl (pH 7.5)
  • 150mM NaCl
  • 1mM Na2EDTA
  • 1mM EGTA
  • 1% Triton
  • 2.5mM sodium pyrophosphate
  • 1mM β-glycerophosphate
  • 1mM Na3VO4
  • 1μg/ml leupeptin

Specifications

Product Type Buffer
Quantity 20 mL
Species Canine, Mouse, Rat, Porcine, Non-Human Primate, Human
For Use With (Equipment) Luminex™ Instruments
Product Line ProcartaPlex

For Research Use Only. Not for use in diagnostic procedures.