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Thermo Scientific™ PNPP Substrates

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

$290.59 - $499.92

Specifications

Product Type ELISA Substrate
Detection Method Colorimetric
Substrate Properties Chemical Substrate
Substrate Type Alkaline Phosphatase
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Products 4
Catalog Number Mfr. No. Description Price Quantity  
Catalog Number Mfr. No. Description Price Quantity  
PI34045
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Thermo Scientific™
34045
PNPP Powder N/A
 
PI34047
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Thermo Scientific™
34047
PNPP Tablets N/A
 
PI37620
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Thermo Scientific™
37620
PNPP Substrate Kit N/A
 
PI37621
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Thermo Scientific™
37621
1-Step™ PNPP Substrate Solution N/A
 
Description

Description

PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.

Features of PNPP (p-nitrophenyl phosphate):
• One-component alkaline phosphatase (AP) substrate
• Absorbance maximum of 405 nm
• Develops a yellow hue in solution upon reaction with AP
• Safer to use—contains a non-mercury, non-azide preservative

The substrate is offered in several formats:
PNPP Substrate
• Powder Form

PNPP Substrate Tablets
• 5 mg tablets with an inert filler as the balance

PNPP Substrate Kit
• PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.

1-Step™ PNPP Substrate Solution
• Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.

Specifications

Specifications

ELISA Substrate
Colorimetric
Chemical Substrate
Alkaline Phosphatase
SDS
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