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pJTI™ R4 EXP CMV-TO EmGFP pA Vector

Catalog No. A15005
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100 μg
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A15005 100 μg
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Catalog No. A15005 Supplier Thermo Fisher Scientific Supplier No. A15005

Description

pJTI™ R4 EXP CMV-TO EmGFP pA Vector

The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green.

Ensure the Success of Your Jump-In™ T-REx™ Retargeting Reactions
Successful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as:

  • Transfection efficiency
  • Cell confluency
  • Antibiotic selection conditions
  • Quality and concentration of DNA
  • Retargeting vector to integrase vector ratio

    We strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.

    • Specifications

    Constitutive or Inducible System Inducible
    Delivery Type Transfection
    Inducing Agent Tetracycline
    Promoter CMV/TO
    Product Type Mammalian Expression Vector
    Reporter Gene GFP (EmGFP)
    Selection Agent (Eukaryotic) Geneticin™ (G-418)
    Content And Storage pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C
    Antibiotic Resistance Bacterial Ampicillin (AmpR)
    Protein Tag GFP (EmGFP)
    Quantity 100 μg
    Vector Jump-In Vectors
    Product Line Jump-In
    For Use With (Application) Stable Cell Line Development, Targeted Integration, Regulated Expression
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    For Research Use Only. Not for use in diagnostic procedures.