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Thermo Scientific™ Pierce™ TMB Substrates

Horseradish peroxidase (HRP) substrates for ELISA that change from blue to yellow upon addition of a sulfuric or phosphoric acid stop solution.

$152.25 - $921.90

Catalog Number Mfr. No. Description Quantity Price Quantity    


thermo scientific™

Substrate Solution, 200mL Stable Peroxide Solution

400mL kit Pack for $234.15


thermo scientific™

Pierce 1-Step Turbo TMB-ELISA Substrate Solution

250mL Pack for $210.00


thermo scientific™

Pierce 1-Step Slow TMB-Elisa Substrate Solution

250mL Pack for $152.25


thermo scientific™
Pierce 1-Step Ultra TMB-ELISA Substrate Solution 250mL Each for $239.40


thermo scientific™
Pierce 1-Step Ultra TMB-ELISA Substrate Solution 1L Each for $921.90
Description & Specifications

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution.

TMB (3,3',5,5' tetramethylbenzidine) is a chromagen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) with major absorbances at 370 and 652nm. The color then changes to yellow (measured at 450nm) upon addition of sulfuric or phosphoric acid to stop the reaction. TMB is very sensitive and more quickly oxidized than other HRP substrates, resulting in faster color development. Unlike other commercially available substrates, these products contain no DMF or DMSO. There are three formulations that differ primarily in their sensitivities. Slow TMB is intermediate in sensitivity, ideal for kinetic readings. The sensitivity of the Turbo TMB compares to that of OPD used at approximately 1mg/mL. Ultra TMB-ELISA produces the highest signal-to-noise ratio and sensitivity in the picogram range.


  • Ready-to-use solutions
  • No hydrogen peroxide required
  • No filtering required
  • Noncarcinogenic
  • Various sensitivities to suit any assay

1-Step TMB Substrates

  • Ready-to-use, one-component substrates
  • Do not require hydrogen peroxide
  • Noncarcinogenic
  • Do not contain DMF or DMSO
  • Available in three formulations that vary by sensitivity

TMB Substrate Kit

  • More sensitive than OPD in endpoint ELISAs
  • Easy to use
  • Provides results in seconds


TMB Substrate Kit (PI-34021): Peroxidase substrate, 200mL; Peroxide solution, 200mL