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Thermo Scientific™ Pierce™ TCEP-HCl, No-Weigh™ Format

Catalog No. PI20490
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10 x 1 mg
1 g
10 g
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Catalog No. Quantity
PI20490 1 g
PI20491 10 g
PIA35349 10 x 1 mg
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Catalog No. PI20490 Supplier Thermo Scientific™ Supplier No. 20490
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Description

Reduce protein and peptide disulfide bonds using this thiol-free, pure, crystalline tris (2-carboxyethyl) phosphine hydrochloride, TCEP (CAS 5961-85-3).

Thermo Scientific™ Pierce TCEP-HCl is pure, crystalline Tris(2-carboxyethyl)phosphine hydrochloride, a thiol-free compound that is highly effective at reducing protein and peptide disulfide bonds, as well as being odorless and stable.

Highlights:

  • Reduces even the most stable water-soluble alkyl disulfides
  • Effective at room temperature and pH 5 in less than five minutes
  • Does not require removal before reaction with maleimide or haloacetyl compounds
  • Water solubility of 310g/L
  • Resistant to air oxidation; nonvolatile and nonreactive toward other functional groups in proteins

Specifications

Content And Storage Upon receipt store at room temperature in sealed container to prevent oxidation. Product shipped at ambient temperature.
Product Line Pierce
Form Solid
Quantity 1 g
Solubility Water
Product Type Disulfide Reducing Reagent

Frequently Asked Questions (FAQs)

Are other TCEP-based products available besides Immobilized TCEP Disulfide Reducing Gel?

Yes. We offer TCEP-HCl (1g solid, Cat. No. 20490, 10g solid, Cat. No. 20491), TCEP-HCl 10 x 1 mg No-weigh format, Cat. No. A35349 and TCEP Solution, Neutral pH (5 mL of stable 500 mM TCEP, Cat. No. 77720).
Can other reducing agents other than DTT or BME be used to reduce proteins prior to electrophoresis? For example, what about TCEP (Tris Carboxy Ethyl Phosphene)?

TCEP, Tris Carboxy Ethyl Phosphene is an alternative sulfhydryl reducing agent for protein samples. It is an extremely potent and effective reducing agent for particularly ‘difficult' proteins. It is compatible with the Tris-Glycine gels and NuPAGE gels. It should be added to the sample buffer for these systems. 20 mM final (maximum) concentration is sufficient for samples. You may add alkylating agents, e.g. Iodine (50 mM Iodoacetic acid), to prevent re-forming of S-S bonds but it is not necessary. Do not heat because this will hydrolyze much of your sample. Instead let the sample sit for several minutes at RT and then load.

For Research Use Only. Not for use in diagnostic procedures.