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Thermo Scientific™ Pierce™ Iodination Beads

Easily and gently label (iodinate) proteins and peptides with iodine-125 using reagent-coated polystyrene beads that can be removed following reaction.

Manufacturer:  Thermo Scientific™ PI28665

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Catalog No. PI28665


 

Specifications

Description

Thermo Scientific™ Pierce Iodination Beads are 3mm diameter polystyrene beads that are coated with an oxidizing reagent, which provides efficient activation of iodine-125 for protein or peptide iodination procedures.

Highlights:

  • Derivatized, uniform, nonporous polystyrene beads
  • Radioiodide incorporation as high as 99%; labeled protein recovery >90%
  • Functions over a broad pH and temperature range
  • Fast and easy to use: iodinations complete in 2 to 15 minutes
  • Reaction stopped by simply removing beads from reaction mixture with tweezers or pasteur pipet; no reducing agent necessary to terminate reaction

Additional Features:

  • Iodinates in the presence of azides, detergents, urea and high salt
  • Allows efficient iodination of cell membrane surface proteins
  • More gentle method for iodination than soluble chloramine-T because there is no contact between the protein and the immobilized oxidizing agent1
  • Highly reproducible iodinations
  • Can be used to quantitatively iodinate histidine at pH 8.22
Description & Specifications

Specifications

Pierce Iodination Beads
Polystyrene beads (3mm dia.) coated with iodination reagent
50 beads
10 to 50 reactions, each with 0.1 to 0.5 mg of protein