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  5. Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media

Cytiva Phenyl Sepharose™ 6 Fast Flow Chromatography Media

Catalog No. 45002940
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Quantity:
25 mL
200 mL
Product Type:
Phenyl Sepharose 6 Fast Flow Media (high sub)
Phenyl Sepharose 6 Fast Flow Media (low sub)
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Catalog No. Quantity Product Type
45002940 25 mL Phenyl Sepharose 6 Fast Flow Media (high sub)
45002937 200 mL Phenyl Sepharose 6 Fast Flow Media (low sub)
45002939 200 mL Phenyl Sepharose 6 Fast Flow Media (high sub)
45002938 25 mL Phenyl Sepharose 6 Fast Flow Media (low sub)
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Catalog No. 45002940 Supplier Cytiva Supplier No. 17097310
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Description

Used for capture and intermediate purification requiring low to medium hydrophobicity

  • Base matrix derivatized via uncharged, chemically-stable ether linkages
  • Two levels of substitution help find optimal selectivity and binding capacity for given application
  • Hydrophilic nature of the base matrix ensures low levels of non-specific binding
  • Hydrophobic interaction chromatography (HIC) media composed of cross-linked 6% agarose beads modified with standard aromatic phenyl groups via uncharged, chemically-stable ether linkages
  • Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development
  • Degree of substitution for low sub HIC media is ∽25μmol phenyl/mL gel
  • Degree of substitution for high sub HIC media is ∽40μmol phenyl/mL gel

Specifications

Quantity 25 mL
For Use With (Application) For Capture and Intermediate PurifiCatIon Requiring Low to Medium Hydrophobicity
Stability Stable in commonly used aqueous Buffers: 1 M NaOH, 3 M Ammonium Sulphate, 30% Isopropanol, 70% Ethanol, 10% Ethylene Glycol, 0.5% SDS, 6 M Guanidine-Hydrochloride, 8 M Urea
Storage Requirements 4°C to 30°C
Product Type Phenyl Sepharose 6 Fast Flow Media (high sub)
Flow Rate 250 to 400 cm/Hoμr
Particle Size 45 to 165 μm
Matrix 6% Cross-Linked Agarose
pH Range 3 to 13
Storage Buffer 20% Ethanol
Ligand Type Phenyl
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