missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Secondary Antibodies
  5. Mouse Secondary Antibodies
  6. 5010789

Invitrogen™ Mouse anti-Rat IgG2c Secondary Antibody, PE, eBioscience™

Catalog No. 5010789
Change view
Click to view available options
Quantity:
100 μg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
5010789 100 μg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 5010789 Supplier Invitrogen™ Supplier No. 12481682
Only null left

Description

Mouse Monoclonal Secondary Antibody

Description: The monoclonal antibody R2C-23A3 recognizes Rat IgG2c antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat or mouse antibody isotypes. Applications Reported: This R2C-23A3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This R2C-23A3 antibody has been tested by flow cytometric analysis of cell stained with rat IgG2c primary antibody. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

Antigen Rat IgG2c
Applications Flow Cytometry
Classification Monoclonal
Clone R2C-23A3
Concentration 0.2 mg/mL
Conjugate PE
Formulation PBS with 0.09% sodium azide; pH 7.2
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Secondary
Target Species Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Secondary Antibody
Form Liquid
Isotype IgG1
Show More Show Less