Invitrogen™ Click-IT™ O-GlcNAc Enzymatic Labeling System

The Click-iT O-GlcNAc Enzymatic Labeling System provides a highly sensitive and efficient method for the in-vitro modification of O-GlcNAc modified proteins. Proteins are enzymatically labeled utilizing the permissive mutant β-1,4-galactosyltransferase (Gal-T1 (Y289L)) which transfers azido-modified galactose (GalNAz) from UDP-GalNAz to O-GlcNAc residues. Then, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT Glycoprotein Detection kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne). Labeling and detection can be completed in less than 24 hours and the Click-iT glycoprotein products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of this important post-translational modification.

• Less hazardous

Cell Analysis, Cell Structure, Click Chemistries, Glycoprotein Analysis with Click Chemistry, Labeling Chemistry, Mitochondria, Mitochondria Related Diseases, Mitochondrial Pathways, Protein and Antibody Labeling, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis