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Applied Biosystems™ MiniAmp™ Thermal Cycler + Plastics

Catalog No. p-7246653
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Description:
Includes 5 x MicroAmp TriFlex 3 x 32-well PCR Reaction Plates (100 plates total)
Includes MicroAmp 8-Tube Strips with Attached Domed Caps, 0.2 mL (125 strips)
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Catalog No. Description
A41194 Includes MicroAmp 8-Tube Strips with Attached Domed Caps, 0.2 mL (125 strips)
A41195 Includes 5 x MicroAmp TriFlex 3 x 32-well PCR Reaction Plates (100 plates total)
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Catalog No. A41194 Supplier Applied Biosystems™ Supplier No. A41194
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The Applied Biosystems MiniAmp Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab’s basic thermal cycling needs.

The Applied Biosystems MiniAmp Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fulfill basic laboratory thermal cycling needs. It has a 5-inch color touchscreen for easy control and may also be accessed through our Thermo Fisher Connect cloud-based platform, providing the freedom to design and securely upload methods from any mobile device or desktop computer.

This MiniAmp Thermal Cycler kit includes a thermal cycler plus either MicroAmp 8-Tube Strips with Attached Domed Caps or MicroAmp TriFlex PCR Reaction Plates with MicroAmp 32-Well Clear Adhesive Films.

MiniAmp Thermal Cycler features

  • Small footprint (19-cm-wide and 39-cm-deep) that can easily fit in almost any lab
  • Front-to-back airflow allows placement of multiple side-by-side units to save valuable bench space
  • 5-inch intuitive color touchscreen for easy programming and training of new users
  • Wi-Fi enabled and Thermo Fisher Connect accessible—offers the freedom to design and securely upload methods from any mobile device or desktop computer

MicroAmp 8-Tube Strips with Attached Domed Caps

MicroAmp 8-Tube Strips with Attached Domed Caps feature individually attached domed caps, dual large end-tabs, and graduated 20-μL markers on each tube to help prevent cross-contamination, pipetting errors, and sample identification errors.

  • Strip of eight tubes with attached caps that open and close independently to prevent contamination and evaporation
  • Angled caps provide efficient tube strip positioning in racks and thermal cycler blocks
  • Etched A-H letter labeling on individual tubes visible when caps are open or closed
  • Dual end tabs for simple labeling and handling
  • Graduated 20-μL measuring markers on each tube for visual volume verification

MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates

MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates have a unique design with three identical polypropylene 32-well PCR plates connected by dual side-tabs for use with 32-well and standard 96-well thermal blocks. These high-quality plates offer thin-wall construction for excellent heat transfer from thermal block to sample and are validated for use with Applied Biosystems PCR thermal cyclers for optimal fit and performance.

  • Designed for use with all Applied Biosystems standard 96-well thermal cyclers
  • Easy-to-tear dual side-tabs for simple separation into three individual 32-well plates
  • Semi-skirted for easy handling and labeling
  • Standard 250 μL working volume, with a 300 μL maximum well volume when used with an adhesive film
  • Alphanumeric labeling on each 32-well plate for easy well identification
  • Certified Human DNA-, DNase-, and RNase-free

MicroAmp 32-Well Clear Adhesive Films

MicroAmp 32-Well Clear Adhesive Film is designed specifically for use with MicroAmp TriFlex 3 x 32-Well PCR Reaction Plates. The film provides an alternative to cap strips and seals 32-well samples securely to prevent PCR sample evaporation and well-to-well contamination.

  • Clear adhesive film for 96-well plates, but pre-cut to fit 32-well plates
  • Easy to apply by simply peeling off the backing and firmly pressing to the plate

Specifications

Thermal Accuracy ±0.25°C (35°C to 99.9°C)
Content And Storage • MiniAmp Thermal Cycler
• 1 x MicroAmp 8-Tube Strips with Attached Domed Caps, 0.2 mL (125 strips)

High-throughput Compatibility Manual Protocols
Thermal Uniformity <0.5°C (20 sec. after reaching 95°C)
Reaction Speed Fast, Standard
Dimensions 15.4 x 7.5 x 7.9 in. (39 x 19 x 20 cm)
Capacity 96-well, 0.2 mL
Voltage 100/240 V
Display Type 5 in. Color TFT LCD Touchscreen
Product Type Thermal Cycler
Warranty Limited Warranty
Thermal Range 0°C to 100°C
User Interface USB, Wi-Fi, Ethernet
Wattage 500 W
Max. Ramp Rate 3°C/sec. (Block), 2.2°C/sec. (Sample)
No. of Programs >1000
Description Includes MicroAmp 8-Tube Strips with Attached Domed Caps, 0.2 mL (125 strips)
Quantity 1 package
Weight 13 lb. (5.9 kg)
Block Configurations 96-well
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What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf