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Applied Biosystems™ MiniAmp™ Plus Thermal Cycler

Catalog No. A37835
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Description

The Applied Biosystems MiniAmp Plus Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab’s basic thermal cycling needs.

The Applied Biosystems MiniAmp Plus Thermal Cycler is a compact, accurate, and economical thermal cycler designed to fit your lab's basic thermal cycling needs. Innovations like a responsive color touch-screen and VeriFlex Blocks temperature-control technology enable simple, accurate optimization. The MiniAmp Plus Thermal Cycler can be accessed through Thermo Fisher Connect, giving you the freedom to design and securely upload your methods from any mobile device or desktop computer.

Key features and benefits:

  • VeriFlex Blocks—with three independent temperature zones for precise temperature control over your PCR optimization
  • 5-inch intuitive color touch-screen—for easy programming and training of new users
  • Small footprint—With dimensions of 19 cm wide and 39 cm deep, you can fit the MiniAmp Thermal Cycler almost anywhere. And because its airflow is front to back, you can set multiple units side by side to save valuable bench space.
  • WiFi-enabled and accessible by Thermo Fisher Connect—giving you the freedom to design and securely upload your methods from any mobile device or desktop computer

Applied Biosystems PCR plastics

Applied Biosystems PCR plastics have been designed and validated to work with our thermal cyclers for more than 25 years. Options for every format and throughput need are available to ensure consistent, precise results for any experiment. To find the best match please visit our Plastics Selection Guide.

Specifications

Thermal Accuracy ±0.25°C (35°C to 99.9°C)
Content And Storage • MiniAmp Thermal Cycler
• Country-specific power cord
High-throughput Compatibility High-throughput Compatible
For Use With (Equipment) Applied Biosystems GeneAmp plastics consumables
Thermal Uniformity <0.5°C (20 sec. after reaching 95°C)
Reaction Speed Fast, Standard
Dimensions 15.4 x 7.5 x 7.9 in. (39 x 19 x 20 cm)
Capacity 96-well, 0.2 mL
Voltage 100/240 V
Display Type 5 in. Color TFT LCD Touchscreen
Product Type Thermal Cycler
Warranty Limited Warranty
Thermal Range 0°C to 100°C
User Interface USB, Wi-Fi, Ethernet
Wattage 500 W
Max. Ramp Rate 3.5°C/sec. (Block), 2.7°C/sec. (Sample)
No. of Programs >1000
Quantity 1 Instrument
Weight 13 lb. (5.9 kg)
Block Configurations 96-well, 3-Zone VeriFlex Block
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Frequently Asked Questions (FAQs)

Why are my PCR tubes crushed/deformed after my PCR run?

The heated thermal cycler lid is designed to make sure that optimized pressure is applied on the microplate for an efficient PCR reaction. When using PCR tubes, excess pressure on the tubes, from the heated lid can cause deformation of the tubes. To avoid this, we recommend using the Tray/Retainer set, as shown below:

Cat. No. 4381850, MicroAmp 96-Well Tray/Retainer Set for Veriti Systems – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the Veriti Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.
Cat. No. 403081, MicroAmp 96-Well Tray/Retainer Set – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes without Caps, 0.2 mL), with the 7000 System, 2720 Thermal Cycler, and GeneAmp PCR System 9700.
Cat. No. 4379983, MicroAmp 96-Well Tray for VeriFlex Blocks – This Tray is for use with MicroAmp Strip Tubes (or MicroAmp Reaction Tubes with Caps, 0.1 mL), with the Veriti Thermal Cycler, Veriti Fast Thermal Cycler, SimpliAmp Thermal Cycler, and Proflex PCR System.

Please take a look at this video: How to Use Adapter Tray Retainers in Thermal Cycler Starter Kits (https://www.youtube.com/watch?v=1uh2ZxZJtgs).
How can I sign in as an administrator to the MiniAmp Plus Thermal Cycler (Cat. No. A37835, A37835, A38076, A38077) and connect to the cloud? What is the default admin password?

The default admin password/PIN is 0000 + the last 4 digits of the instrument serial number. For example, if the serial number was 1101234567, then the default admin password would be 00004567.

What is the password for the MiniAmp Plus Thermal Cycler

The password for the MiniAmp Plus Thermal Cycler is 0000 plus the last four digits of the instrument serial number. For example, if the instrument serial number is 123456789 then the password would be (00006789).
Do you offer an adapter to fit 96-well skirted plates with the MiniAmp Plus Thermal Cycler (Cat. No. A37835)?

Unfortunately, we do not offer an adapter for using skirted plates with the MiniAmp Plus Thermal Cycler (Cat. No. A37835).

Please use our PCR and qPCR Plastics Selection Tool to find compatible plates and accessories for your instrument.

What is the optimal ramp rate for my thermal cycler?

There is no universal ramp rate for a thermal cycler because both the block and sample ramp rates are different for each thermal cycler. The sample ramp rate changes with the volume of the sample since more time and energy are required to heat higher sample volumes. The ramp rate may also need to be changed for a particular PCR assay or primer pair if you are trying to use the same PCR assay on two different thermal cyclers, for example.

As a reference, the block ramp rate commonly used with DreamTaq Polymerase on the MiniAmp thermal cycler is 3.0 C/sec. In a 50 µL PCR reaction, this translates to a sample ramp rate of ~1 C/sec.

Please refer to the following application note for further information about thermocycler ramp rates: https://assets.thermofisher.com/TFS-Assets/LSG/brochures/Thermal_Cycler_Ramp_RatesAppNote.pdf

What is the difference between the MiniAmp and MiniAmp Plus Thermal Cyclers?

There are 2 key differences:
- The MiniAmp Plus Thermal Cycler has 3 VeriFlex zones while the MiniAmp Thermal Cycler does not.
- The MiniAmp Plus Thermal Cycler has a block ramp rate of 3.5 degrees C/sec while the MiniAmp Thermal Cycler has a block ramp rate of 3.0 degrees C/sec.
Is there wireless connectivity with the MiniAmp/MiniAmpPlus Thermal Cycler?

Like the ProFlex PCR System and SimpliAmp Thermal Cycler, the MiniAmp/MiniAmpPlus Thermal Cycler can be connected wirelessly with an optional High-Power USB W-Fi Module, Cat. No. A26774.
How do I program an infinite hold in the MiniAmp/MiniAmpPlus Thermal Cycler?

To add an infinite hold, select the infinity symbol for program.
How do the VeriFlex Blocks work on the MiniAmp Plus Thermal Cycler?

The MiniAmp Plus Thermal Cycler features innovative VeriFlex Blocks for enhanced PCR functionality. Three separate Peltier blocks (4 columns each) provide maximal versatility and flexibility and offer two key benefits:

- PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
- Run more experiments: the three Peltier blocks can also be used to run up to three different annealing temperatures in the same run.

What is the Auto Delta feature on the MiniAmp/MiniAmpPlus Thermal Cycler?

The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.
What is the maximum block ramp rate of the MiniAmp/MiniAmp Plus Thermal Cycler?

The maximum block ramp rate of the MiniAmp Thermal Cycler is 3 degrees C/sec.
The maximum block ramp rate of the MiniAmp Plus Thermal Cycler is 3.5 degrees C/sec.
What types of plastics can I use with the MiniAmp/MiniAmp Plus Thermal Cycler?

The compatible plates and plastics are the same as those used with the ProFlex 96-Well PCR System and can be viewed here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/thermal-cycler-plastics/proflex-96-3x32.html).
What types of blocks are available on the MiniAmp/MiniAmp Plus Thermal Cycler?

We offer only a 96-well standard (0.2 mL) block that is not interchangeable. The 96-well block for the MiniAmp Plus Thermal Cycler has a block with 3 VeriFlex zones while the MiniAmp Thermal Cycler has an isothermal block.

For Research Use Only. Not for use in diagnostic procedures.