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  5. Thermo Scientific™ Klenow Fragment (10 U/μL)

Thermo Scientific™ Klenow Fragment (10 U/μL)

Catalog No. FEREP0051
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Quantity:
1,500 units
300 units
Concentration:
10 U/μL
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Catalog No. Quantity Concentration
FEREP0051 300 units 10 U/μL
FEREP0052 1,500 units 10 U/μL
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Catalog No. FEREP0051 Supplier Thermo Scientific™ Supplier No. EP0051
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Description

Klenow Fragment is the Large Fragment of DNA Polymerase exhibiting 5'→3' polymerase, 3'→5' exonuclease (proofreading) activities, but lacking 5'→3' exonuclease activity.

Thermo Scientific Klenow Fragment is the large fragment of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks 5'→3' exonuclease activity of DNA polymerase I.

Highlights

  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, aminoallyl-, biotin-, digoxigenin- and fluorescently-labeled nucleotides)
  • Active in restriction enzyme, PCR, RT, and T4 DNA Ligase buffers

Applications

  • DNA blunting by fill-in 3'-overhangs
  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • DNA sequencing by the Sanger method
  • Site-specific mutagenesis of DNA with synthetic oligonucleotides
  • Second strand synthesis of cDNA

Specifications

Concentration 10 U/μL
Polymerase DNA Polymerase I
Quantity 300 units
Product Type Klenow Fragment

For Research Use Only. Not for use in diagnostic procedures.