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Invitrogen™ pYES2.1 TOPO™ TA Yeast Expression Kit
Offers direct cloning of Taq-amplified PCR product into a Saccharomyces cerevisiae expression vector
Supplier: Invitrogen™ K415001
Includes: Each pYES2.1 TOPO TA Expression Kit contains two boxes. The pYES2.1 TOPO TA box contains all of the reagents necessary for ligation, including 200ng of topoisomerase I-activated pYES2.1/V5-His-TOPO vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, GAL1 Forward and V5 C-term Reverse Primers for sequencing or PCR screening, and an expression control plasmid. The other box contains all of the reagents necessary for transformation, including 21 x 50μL aliquots of chemically competent TOP10F' E. coli, S.O.C. medium, and supercoiled plasmid control pUC19.
Description
- Uses linearized, topoisomerase I-activated pYES2.1/V5-His-TOPO vector for 5-minute cloning on bench top and produces >85% recombinants
- pYES2.1/V5- His-TOPO vector features 2μ origin of replication for high-copy maintenance gene for auxotrophic selection on economical minimal media
- C-terminal V5 epitope and polyhistidine (6xHis) tags for efficient detection and purification
Protein Expression, Proteins, Expression, Isolation and Analysis, S. cerevisiae Expression Systems, Yeast Expression
Specifications
| GAL1 | |
| Each pYES2.1 TOPO™ TA Expression Kit contains two boxes. The pYES2.1 TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pYES2.1/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, GAL1 Forward and V5 C-term Reverse Primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Box contains all of the reagents necessary for transformation, including twenty-one 50 μl aliquots of chemically competent TOP10F´ E. coli, S.O.C. medium, and supercoiled plasmid control pUC19. Store at -80°C. All components are guaranteed stable at the indicated temperature for 6 months when properly stored. | |
| His Tag (6x), V5 Epitope Tag | |
| 20 reactions | |
| TOPO, YES |
| TA Yeast Expression Kit | |
| Ampicillin (AmpR) | |
| TOPO-TA | |
| pYES, TOPO-TA Cloning Vectors |
For Research Use Only. Not for use in diagnostic procedures.