missing translation for 'onlineSavingsMsg'
Learn More
Please login to your online account to display your discounted pricing

Invitrogen™ pPICZ A, B, & C Pichia Vectors DFS Item

Designed for simple cloning and selection, high-level expression, and rapid detection and purification of the recombinant protein

Supplier:  Invitrogen™ V19020

Catalog No. V19020



Includes: The EasySelect PichiaExpression Kit includes 20μg each of pPICZ A, B, and C and pPICZ A, B, and C, Pichia pastorisstrains X-33 (wild-type), GS115 (his4), KM71H (arg4 aox1::ARG4), and control strains for both intracellular and secreted expression, 250mg Zeocin , media (YP and YNB), EasyComp PichiaTransformation Kit, 2μg each 5' AOX1, 3' AOX1,and α-factor sequencing primers. A lacZ positive expression control is provided with pPICZ.

Description

Description

  • These vectors contain Zeocin™ resistance gene for direct selection of multi-copy integrant strains
  • By selecting with increasing amounts of Zeocin, strains with multiple copies of the gene of interest integrated into genome are obtained
  • Increasing number of copies of gene of interest in recombinant Pichiastrain can result in higher expression levels
  • Inducible AOX1promoter for high-level expression in Pichia pastoris c-mycepitope tag for convenient detection with Anti-mycAntibody
  • C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with Anti-His(C-term) Antibody
  • The pPICZ vector also features secretion signal for efficient transport of proteins to medium

Pichia pastoris Expression Systems, Protein Expression, Proteins, Expression, Isolation and Analysis, Yeast Expression

Specifications

Specifications

AOX1
20 μg each of pPICZ A, B, & C suspended in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. A GS115/pPICZ/lacZ Positive Control strain is also included.

Store vectors at -5 to -30°C.
His Tag (6x), c-Myc Epitope Tag
pPIC
Pichia Expression Vector
Zeocin™ (ZeoR)
Restriction Enzyme/MCS
SDS
Documents

Documents

Promotions

Promotions

For Research Use Only. Not for use in diagnostic procedures.