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Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli
Description
Includes
MultiShot TOP10 Chemically Competent E. coli: Five 96-well plates, 15μL per well, pUC19 DNA (10pg/μL): 1 vial, 50μL, S.O.C. medium: 5 bottles, 15mL each
MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains. Packaged in five 96-well microtiter plates (15 μL aliquots) to simplify high-throughput workflows and provide transformation efficiencies of >1 x 108 cfu/μg control plasmid DNA, Multishot TOP10 is a versatile strain that can be used in multiple routine cloning applications.
Benefits of MultiShot TOP10 competent cells
• TOP10 strain is ideal for high-efficiency cloning and plasmid propagation
• Microtiter plate format design is convenient for automated high-throughput cloning
• mcrA mutation supports efficient transfection of methylated DNA
• lacZΔM15 allele permits rapid blue/white screening of transfectants
• endA1 mutation reduce endonuclease activity and improve plasmid DNA yields
• recA1 mutation helps reduce unwanted recombination
Easy to use for high-throughput transformations
MultiShot TOP10 Chemically Competent cells in 96-well plates are designed to facilitate high-throughput transformation. This format allows all steps of the transformation protocol, up to plating, to take place in the same well, saving time and reducing the chance of contamination. After the addition of DNA, MultiShot TOP10 Chemically Competent cells can be transformed by heat-shock at 42°C using a heat block or thermocycler in the same plate.
Using our TA Cloning and TOPO Cloning protocols, including controls, yields of 100–400 colonies per agar plate can be expected.
Genotype
F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) endA1 nupG
Find the strain and format that fits your needs
• DH strains are available in chemically competent and electrocompetent cell formats.
• For high-throughput transformation options, choose from our collection of MultiShot formatted comp cells.
Order Info
Shipping Condition: Dry Ice
Specifications
Specifications
| Product Type | Chemically Competent Cells |
| Contains F' Episome | No |
| Improves Plasmid Quality | Yes (endA1) |
| Cloning Methylated DNA | Yes (mcrA) |
| Transformation Efficiency Level | Medium Efficiency (1 x 108 to 1 x 109 cfu/μg) |
| Content And Storage | • MultiShot™ TOP10 Chemically Competent E. coli (5 x 96-well plates at 15 μL per well) Store Competent Cells at –80°C. • pUC19 DNA (1 x 50 μL) Store pUC19 DNA at –20°C. • S.O.C. medium (5 x 15 mL/bottle) Store S.O.C. Medium at 4°C or at room temperature |
| Antibiotic Resistance Bacterial | Yes (Streptomycin) |
| Cloning Unstable DNA | Not suitable for cloning unstable DNA |
| Blue/White Screening | Yes (lacZΔM15) |
| High-throughput Compatibility | High |
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Frequently Asked Questions (FAQs)
The MultiShot StripWell format is supplied as pre-aliquoted competent cells in 96 tubes contained in a rack. The tubes are attached in strips of 8 tubes, and there are 12 strips that contain 50 µL of chemically competent cells. This format offers the most flexibility to transform 1 tube or up to all 96 tubes at once and is intended for varied or medium-throughput use.
The MultiShot FlexPlate format is supplied as pre-aliquoted competent cells in a 96-well PCR plate. Each well contains 20 µL of competent cells. The plate can be separated at each column of 8 wells, offering a high level of flexibility to utilize various increments of 8 wells per transformation. The wells of the plate are heat sealed, preventing detachment of the aluminum seal in the -80 degrees C freezer and preventing contamination. This format is compatible with automated liquid handling platforms and is intended for medium- to high-throughput use.
The MultiShot format is supplied as pre-aliquoted competent cells in 5, 96-well thin-wall polycarbonate microplates. Each well of the 96-well microplate contains 15 µL of chemically competent cells. This format is compatible with automated liquid handling platforms and is intended for high-throughput use.
For Research Use Only. Not for use in diagnostic procedures.