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Invitrogen™ MaV203 Competent Yeast Cells, Subcloning Scale

Catalog No. 11445012
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11445012 4 x 0.1 mL
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Catalog No. 11445012 Supplier Invitrogen™ Supplier No. 11445012

Description

Designed for use with ProQuest™ Two-Hybrid System

  • S. cerevisiae strain MaV203 contains deletions in endogenous GAL4 and GAL80 genes for use with GAL4-based two-hybrid systems
  • Has leu2 and trp1 mutations for selection of ProQuest bait and prey vectors
  • MaV203 contains three GAL4 inducible reporter genes, providing four phenotypes, for easier identification of true interactors
  • Transformation (250μL of cells) efficiency yields >1 x 106 colonies
  • Control DNA pMAB12 to confirm transformation efficiency and preparation of selection media
  • Pre-qualified PEG/Lithium Acetate to maximize number of trans-formants
  • Competent cells formatted with carrier DNA already included.
  • Lacks F' episome
  • Chemically competent

Not high throughput compatible (manual)

Protein Expression, Protein-Protein Interactions, Proteins, Expression, Isolation and Analysis, S. cerevisiae Expression Systems, Yeast Expression, Yeast Two-Hybrid

Order Info

Shipping Condition: Dry Ice

Specifications

Format Tube(s)
Product Type Yeast Cells
Content And Storage Store in ultra-cold freezer (-68 to -85°C).
Bacterial or Yeast Strain MaV203
Blue/White Screening No
High-throughput Compatibility Not High-throughput Compatible (Manual)
Improves Plasmid Quality No
Species S. cerevisiae
Shipping Condition Dry Ice
Quantity 4 x 0.1 mL
Product Line ProQuest
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Frequently Asked Questions (FAQs)

Does the ProQuest Two-Hybrid System yeast strain, MaV203 have slow growth compared to other commonly used laboratory Saccharomyces cerevisiae strains?

To avoid interference of endogenous GAL4 and GAL80 proteins, MaV203 must carry deletions of the GAL4 and GAL80 genes. As a result of deletion of these two genes, MaV203 cells grow more slowly compared to yeast strains containing the wild-type version of these genes. Please note that the growth rate will depend on the type of growth medium used (e.g., YPD versus SC drop-out media).
Are the MaV203 yeast cells that come with the ProQuest Two-Hybrid System ready for transformation?

No, the MaV203 cells are provided as a glycerol stock. Cells from this stock need to be streaked on a YPD plate for growth at 30 degrees C. Resulting colonies can be used to prepare competent cells for transformation as per the ProQuest Two-Hybrid System manual. Alternatively, ready-to-use MaV203 Competent Yeast Cells, Subcloning Scale (Cat. No. 11445-012) or Library Scale (Cat. No. 11281-011) are available for purchase from Thermo Fisher Scientific.
I sequenced one of your vectors after PCR amplification and observed a difference from what is provided online (or in the manual). Should I be concerned?

Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.

Are your vectors routinely sequenced?

No, our vectors are not routinely sequenced. Quality control and release criteria utilize other methods.

How was the reference sequence for your vectors created?

Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources.

For Research Use Only. Not for use in diagnostic procedures.