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Invitrogen™ Flp-In™-CHO Cell Line

Catalog No. R75807
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R75807 1 mL
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Catalog No. R75807 Supplier Invitrogen™ Supplier No. R75807
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Description

Includes

3 x 106 cells are supplied frozen in 1mL 90% complete medium and 10% DMSO.

Designed for rapid generation of stable cell lines that express protein of interest from Flp-In expression vector

  • These cells contain single stably integrated FRT site at transcriptionally active genomic locus
  • Targeted integration of Flp-Inexpression vector ensures high-level expression of gene of interest
  • Created by transfecting parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™-resistant clones

  • The Flp-In-CHO Cell Line was created by transfecting CHO cells with pFRT/lacZeo2 and selecting for Zeocin™-resistant clones
  • Flp-In-CHO cell lines work well with Flp-In vectors that express gene from CMV promoter (pcDNA™5/FRT, pcDNA5/FRT/V5-His-TOPO™, and pSecTag/FRT/V5-His-TOPO™)

Constitutive Expression, Mammalian Expression, Mammalian Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration

Specifications

Content And Storage 3 x 106 cells are supplied frozen in 1 ml of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.
Cell Line Flp-In™-CHO Cell Line
Species Hamster
Protein Tag pSec
Quantity 1 mL
Product Line Flp-In

For Research Use Only. Not for use in diagnostic procedures.