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Invitrogen™ Flp-In™-3T3 Cell Line

Catalog No. R76107
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R76107 1 mL
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Catalog No. R76107 Supplier Invitrogen™ Supplier No. R76107
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Description

Includes

3 x 106 cells are supplied frozen in 1mL 90% complete medium and 10% DMSO.

Designed for rapid generation of stable cell lines that express protein of interest from Flp-In expression vector

  • These cells contain single stably integrated FRT site at transcriptionally active genomic locus
  • Targeted integration of Flp-Inexpression vector ensures high-level expression of gene of interest
  • Created by transfecting parent cell lines with pFRT/lacZeo and selecting for stable Zeocin™-resistant clones
  • Flp-In-3T3 cells tend to down regulate CMV promoter

Constitutive Expression, Mammalian Expression, Mammalian Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration

Specifications

Content And Storage 3 x 106 cells are supplied frozen in 1 ml of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.
Cell Line Flp-In™-3T3
Species Mouse
Protein Tag pSec
Quantity 1 mL
Product Line Flp-In

Frequently Asked Questions (FAQs)

Is multiple integration of the Flp-In expression construct possible? How do you screen for multiple integrants, and how stable is the Flp-In expression cell line?

In theory, one can get multiple integrations of the Flp-In expression construct—an FRT-specific integration event and a random, second-site integration. However, random integration is a relatively uncommon event. Limiting the amount of DNA in the transfection will reduce the chance of second-site integration. We have transfected 293 cells (lacking the FRT site) with the pcDNA5/FRT vector and have identified one potential second-site integrant after screening over 200 clones. DNA integrations can be detected by Southern blot. A single integrant will display a single band; double: two; triple: three, etc. We have maintained a number of Flp-In expression cell lines for over four months and have not observed any loss of the Flp-In expression construct, whether hygromycin selection was maintained or not.
What kind of Flp-In T-REx cell lines do you offer?

We offer the Flp-In T-REx system that contains pFRT/lacZeo and pcDNA6/TR stably integrated into HEK 293 cells. This cell line has been functionally tested for its ability to regulate expression.

For Research Use Only. Not for use in diagnostic procedures.