Learn More
Invitrogen™ LR Clonase™ II Plus enzyme
Description
Includes
Proteinase K solution (2μg/μL) and a positive control vector
Gateway™ LR Clonase™ enzyme mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor) and Xis (Excisionase) enzymes that catalyze the in vitro recombination between an Entry clone (containing a gene of interest flanked by attL sites) and a Destination vector (containing attR sites) to generate your expression clone. We offer different formats of LR Clonase enzyme mixtures, depending on your application and desired format.
LR Clonase II Plus enzyme mix is the latest version that offers the highest recombination efficiency available(Table 1), and is specifically optimized for both single and multiple fragment cloning (Table 1). LR Clonase II Plus enzyme mix is provided in an optimized single mix of enzyme and reaction buffer, ensuring enzyme stability and easy of use with few pippetting steps for both single and multiple fragment cloning. Our LR Clonase II enzyme mix is also available in a single mix format, while the original LR Clonase® enzyme is the enzyme and buffer in separate tubes.
Order Info
Shipping Conditions: Dry ice
Specifications
Specifications
| Content And Storage | Includes LR Clonase II Plus enzyme and proteinase K solution (2 μg/μL). Store at -80°C. Guaranteed stable for 6 months when properly stored. |
| Shipping Condition | Dry Ice |
| Enzyme | LR Clonase |
| Compatible Buffer | Reaction Buffer |
| Quantity | 100 Reactions |
| Product Type | LR Clonase Enzyme |
| Product Line | Clonase, Gateway |
Frequently Asked Questions (FAQs)
Gateway Cloning Technology is an easy-to-use system for cloning and subcloning DNA segments (e.g. genes of interest), facilitating gene functional analysis, protein expression, and the integration of technology platforms. One can also readily clone PCR products into so-called Gateway "Entry" vectors. To shuttle inserts from one vector to another, the Gateway Cloning Technology uses bacteriophage lambda-based site-specific recombination. There is no need to use restriction enzymes and ligase to subclone inserts.
One advantage of Gateway Cloning Technology is that genes present in a single Gateway Entry vector can be subcloned into multiple different Gateway Destination vectors. After this 1 hour in vitro subcloning reaction, a high percentage of the colonies obtained carry the desired expression clone. For more details, please see the product manual for cat# 12535029 or cat# 12535037.
For Research Use Only. Not for use in diagnostic procedures.