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Invitrogen™ Biotin-14-dCTP

Catalog No. 19518018
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50 nmol
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19518018 50 nmol
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Catalog No. 19518018 Supplier Invitrogen™ Supplier No. 19518018
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Description

Includes

Biotin-14-dCTP is supplied at 0.4mM in 100mM Tris-HCl (pH 7.5), 0.1mM EDTA.

Used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling

About Biotin-14-dCTP
Biotin-14-dCTP is a patented dCTP analog with biotin attached at the N4-position of the pyrimidine base by a 14-atom linker. This nucleotide can be efficiently incorporated into DNA by nick translation in the presence of dATP, dGTP, and dTTP, labeling DNA with terminal deoxynucleotide transferase, or random primer synthesis. The amount of material provided is sufficient for labeling up to 50μg of DNA.

Performance and quality testing
Purity is evaluated by reverse-phase HPLC.

  • Convenience and safety: probe preparation generates no radioactive waste
  • Stability: probes remain active longer than isotopically labeled probes
  • Versatility: biotinylated DNA can be used for immobilization and capture procedures via streptavidin binding

DNA and RNA Purification and Analysis, DNA Labeling, Nucleic Acid Labeling and Oligo Synthesis

Order Info

Shipping Condition: Dry Ice

Specifications

Product Type Labeled Nucleotide
Concentration 0.4 mM
Content And Storage Biotin-14-dCTP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.
Label or Dye Biotin & Other Hapten
Labeling Method Direct Labeling
Shipping Condition Dry Ice
Quantity 50 nmol
Form Liquid

For Research Use Only. Not for use in diagnostic procedures.