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  5. Cytiva IMAC Sepharose™ 6 Fast Flow Affinity Media

Cytiva IMAC Sepharose™ 6 Fast Flow Affinity Media

Catalog No. 45002967
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Quantity:
25 mL
100 mL
Particle Size:
45 to 165 μm
90 μm
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Catalog No. Quantity Particle Size
45002967 100 mL 45 to 165 μm
45002966 25 mL 90 μm
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Catalog No. 45002967 Supplier Cytiva Supplier No. 17092108
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Description

Used for purifying proteins and peptides that have an affinity for metal ions. Cytiva IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.

  • Uncharged IMAC resin allows possibility to charge with metal ion of choice for optimized selectivity
  • Used for ions such as histidine-tagged proteins but also untagged recombinant or native proteins
  • High protein binding capacity
  • Coupling chemistry and novel ligand designed to give minimal ligand leakage
  • Available HiTrap™ and HiPrep™ pre-packed formats for easy scale-up and process development

 

Specifications

Quantity 100 mL
For Use With (Application) For Purifying Proteins and Peptides That Have an Affinity for Metal Ions
Stability Stable in commonly used aqueous Buffers: 0.01 M HCl, 1 M NaOH, 70% Acetic Acid, 2% SDS, 30% 2-Propanol
Storage Requirements 4°C to 30°C
Product Type IMAC Sepharose 6 Fast Flow Media
Flow Rate 250 to 400 cm/Hoμr
Particle Size 45 to 165 μm
Height (English) Bed 9.84 in.
Height (Metric) Bed 25 cm
Max. Pressure 100 kPa
Matrix 6% Cross-Linked Agarose
pH Range 3 to 12
Storage Buffer 20% Ethanol
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