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Invitrogen™ Human PNP ELISA Kit

Numéro de catalogue. EH373RBX5
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5 x 96 Tests
96 Tests
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EH373RBX5 5 x 96 Tests
EH373RB 96 Tests
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Numéro de catalogue. EH373RBX5 Fournisseur Invitrogen™ Code fournisseur EH373RBX5
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ELISA

Human PNP quantitates human PNP in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human PNP.

This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants.

Spécifications

Accession Number AL024301; AU015798; epididymis secretory sperm binding protein Li 156 an; HEL-S-156 an; inosine phosphorylase; inosine phosphorylase {ECO:0000250; Inosine-guanosine phosphorylase; NP; Np-1; Np-2; nucleoside phosphorylase; PNP; PNP {ECO:0000250; Pnp1; PRO1837; PUNP; Purine nucleoside phosphorylase; purine nucleoside phosphorylase {ECO:0000250; purine-nucleoside phosphorylase; purine-nucleoside phosphorylase 1; purine-nucleoside:orthophosphate ribosyltransferase; UniProtKB:P00491}
Assay Range 0.05 to 10 ng/mL
Assay Sensitivity 0.05 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 4860
Gene Symbol PNP
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target PNP
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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