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  5. Invitrogen™ Human Ficolin-3 ELISA Kit

Invitrogen™ Human Ficolin-3 ELISA Kit

Numéro de catalogue. EH193RBX5
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5 x 96 Tests
96 Tests
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Numéro de catalogue. Quantity
EH193RBX5 5 x 96 Tests
EH193RB 96 Tests
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Numéro de catalogue. EH193RBX5 Fournisseur Invitrogen™ Code fournisseur EH193RBX5
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Description

ELISA

Human Ficolin-3 quantitates human Ficolin-3 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant human Ficolin-3.

H-Ficolin (Hakata antigen, Ficolin-3) is an innate immunity pattern recognition molecule found in human serum. H-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP) formed by carbohydrates (GlcNAc, GalNAc and fucose) activating complement through the lectin pathway by means of the associated MASPs ((MBL)-associated serine proteases). H-Ficolin consists of 35 kDa protein chains associated in triplet subunits which are again associated in high molecular weight oligomers by means of disulfide bonds at the N-terminal region.

Spécifications

Accession Number Collagen/fibrinogen domain-containing lectin 3 p35; Collagen/fibrinogen domain-containing protein 3; FCN3; FCNH; ficolin 3; Ficolin-3; HAKA1; Hakata antigen; MGC22543
Assay Range 0.25 to 50 ng/mL
Assay Sensitivity 0.25 ng/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 8547
Gene Symbol FCN3
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 5 x 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target Ficolin 3
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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