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Gibco™ Human FGF-18, Animal-Free Recombinant Protein, PeproTech®

Numéro de catalogue. AF10028250U
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250 μg
5 μg
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Numéro de catalogue. Quantity
AF10028250U 250 μg
AF100285UG 5 μg
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Numéro de catalogue. AF10028250U Fournisseur Gibco™ Code fournisseur AF10028250UG
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Description

Recombinant Protein

Recombinant Human FGF-18 derived from E.coli is a 20.1 kDa protein consisting of 173 amino acid residues, resulting from C-terminal truncation of the full length protein. This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

FGF18 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures.

Spécifications

Accession Number O76093
For Use With (Application) Functional Assay
Format Lyophilized
Formulation protein with no preservative
Gene ID (Entrez) 8817
Molecular Weight (g/mol) 20.1 kDa
Name Human FGF-18, Animal-Free
Purification Method HPLC, SDS-PAGE
Quantity 250 μg
Regulatory Status RUO
Endotoxin Concentration <0.1 EU/ μg
Gene Alias D130055P09Rik; FGF; FGF18; FGF-18; Fibroblast growth factor; Fibroblast growth factor 18; UNQ420/PRO856; zFGF5
Common Name FGF18
Gene Symbol Fgf18
Biological Activity Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 10^5 units/mg
Product Type Protein
Conjugate Unconjugated
Recombinant Recombinant
Sequence AEENVDFRIH VENQTRARDD VSRKQLRLYQ LYSRTSGKHI QVLGRRISAR GEDGDKYAQL LVETDTFGSQ VRIKGKETEF YLCMNRKGKL VGKPDGTSKE CVFIEKVLEN NYTALMSAKY SGWYVGFTKK GRPRKGPKTR ENQQDVHFMK RYPKGQPELQ KPFKYTTVTK RSR
Content And Storage -20°C
Activity Determined by a cell proliferation assay using Balb/c 3T3 cells. The expected ED50 is ≤ 10 ng/ml, corresponding to a specific activity of ≥ 1 x 10^5 units/mg
Expression System E. coli
Form Lyophilized
Purity or Quality Grade ≥ 95% by SDS-PAGE gel and HPLC analyses.
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