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Cytiva HiTrap™ AVB Sepharose™ Columns

Catalog No. 4520060
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Quantity:
1 x 5 mL
5 x 1 mL
Diameter (Metric) Inner:
7 mm
16 mm
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Catalog No. Quantity Diameter (Metric) Inner
4520060 1 x 5 mL 16 mm
4520059 5 x 1 mL 7 mm
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Catalog No. 4520060 Supplier Cytiva Supplier No. 28411212
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Description

Enables purification of adeno associated viruses (AAV) in one step, with high purity and excellent yields. Cytiva HiTrap™ AVB Sepharose™ Columns are pre-packed with affinity chromatography medium (resin) for simple operation with a syringe, pump or chromatographic system.

AVB Sepharose High Performance is an affinity resin designed for the purification of adeno associated virus (AAV). It enables purification of adeno associated viruses in one step with high purity and excellent yields.
  • One step purification of adeno associated viruses
  • Affinity resin with high selectivity and low background binding
  • Large surface area to give enhanced capacity
  • Prepacked HiTrap columns for simple operation with a syringe, pump, or chromatographic system
AVB Sepharose High Performance has proven affinity for adeno associated viruses from subclasses 1, 2, 3, and 5.
  • It is expected to have affinity for most of the other adeno associated virus subclasses
  • The ligand is a 14 kD fragment from a single chain antibody, expressed in yeast

Specifications

Quantity 1 x 5 mL
For Use With (Application) One Step Purification of Adeno Associated Viruses
Stability Stable to all commonly used aqueous buffers
Storage Requirements Store prepacked column at 4°C to 30°C in 20% Ethanol and Media at 4°C to 8°C in 20% Ethanol.
Binding Capacity >10▿12 genecopies/mL Chromatography Mediμm
Product Type HiTrap AVB Sepharose HP Column
Diameter (Metric) Inner 16 mm
Flow Rate <150 cm/hr
Particle Size 24 to 44 μm
Height (Metric) Bed 25 mm
Material Polypropylene
Max. Pressure 70 psi (5 bar)
Matrix Cross-linked agarose
pH Range 3 to 10
Bed Volume (Metric) 5 mL
Ligand Type 14 kD recombinant protein
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