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Gibco™ FreeStyle™ CHO Expression Medium
Description
FreeStyle CHO Expression Medium is specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation. This medium requires supplementation with 8 mM glutamine before use.
- Formulation without hypoxanthine and thymidine for use with or without dhfr systems
- Ability to support high-density suspension growth and transient transfection of CHO-S cells
- Animal origin-free, protein-free and chemically defined formulation
- Easy use as part of the FreeStyle MAX CHO Expression System for protein production
- Scalability in spinner flasks and bioreactors
Formulation without hypoxanthine and thymidine for use with or without dhfr systems
- Gibco FreeStyle CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems
- Non-dhfr systems require the addition of HT Supplement before use
- Gibco FreeStyle CHO cells in suspension can be cultured directly into Gibco FreeStyle CHO Expression Medium without adaptation
- Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco FreeStyle CHO Expression Medium, usually without any further adaptation
- Cells usually require adaptation from serum-containing formulations
- Cells cultured in Gibco FreeStyle CHO Expression Medium can be transfected using lipid-based transfection reagents, such as FreeStyle MAX reagent
- Gibco FreeStyle CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest
- Gibco chemically defined media contain no proteins, hydrolysates, or components of unknown composition
- Protein production using Gibco FreeStyle CHO Expression Medium can be scaled up in spinner flasks or bioreactors
- The appropriate spinner or impeller speed and seeding density should be optimized for each system
- Depending on the impeller design and speed, it may be necessary to supplement Gibco FreeStyle CHO Expression Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture
Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture
Order Info
Shipping Condition: Room temperature
Compliance
Gibco FreeStyle CHO Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
Specifications
| Product Type | Expression Medium |
| Cell Line | FreeStyle™ CHO-S Cells, CHO |
| Content And Storage | Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
| Classification | Chemically Defined, Protein-Free, Serum-Free |
| Culture Type | Suspension Cell Culture |
| Endotoxin Level | ≤0.25 EU/mL, ≤ 0.25 EU/mL |
| Glutamine | No Glutamine |
| Phenol Red Indicator | No Phenol Red |
| Serum Level | Serum-Free |
| Shipping Condition | Room Temperature |
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Frequently Asked Questions (FAQs)
With FreeStyle 293 Expression Medium, we would recommend harvesting the cells a little earlier to reduce any nonspecific binding by host cell proteins and then filtering the culture supernatant through a 0.2 or 0.1 µM membrane before loading onto the column. On the other hand, FreeStyle CHO Expression Medium contains EDTA that will strip the nickel column. The medium can be subjected to dialysis or buffer exchange prior to loading on the column, or the EDTA can be chelated by adding 1-3 mg/L NiSO4 or NiCl to the supernatant. Iron or cobalt salts can also be used.
When cells meant to be grown in suspension are grown in static culture, they may form clumps. These clumps will severely limit transfection efficiency and protein expression. It is suggested that FreeStyle 293 cells in FreeStyle media and CHO-S cells in CD-CHO or CHO-SFM are grown in agitated suspension to reduce the appearance of clumps. However, if clumps do form, you can try the following protocol to select for cells that don't form clumps:
- Transfer cells into an appropriate size centrifuge tube that will hold the entire cell suspension.
- Allow cells to sit undisturbed for about 5 minutes. The time can vary depending on the specific cell line. Larger cell clumps will settle to the bottom of the tube.
- Collect cells from the upper portion of the tube to passage into a new flask.
For Research Use Only. Not for use in diagnostic procedures.