Gibco™ FreeStyle™ CHO Expression Medium

FreeStyle CHO Expression Medium is specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation. This medium requires supplementation with 8 mM glutamine before use.

• Formulation without hypoxanthine and thymidine for use with or without dhfr systems
• Ability to support high-density suspension growth and transient transfection of CHO-S cells
• Animal origin-free, protein-free and chemically defined formulation
• Easy use as part of the FreeStyle MAX CHO Expression System for protein production
• Scalability in spinner flasks and bioreactors

Formulation without hypoxanthine and thymidine for use with or without dhfr systems

• Gibco FreeStyle CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems
• Non-dhfr systems require the addition of HT Supplement before use

• Gibco FreeStyle CHO cells in suspension can be cultured directly into Gibco FreeStyle CHO Expression Medium without adaptation
• Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco FreeStyle CHO Expression Medium, usually without any further adaptation
• Cells usually require adaptation from serum-containing formulations
• Cells cultured in Gibco FreeStyle CHO Expression Medium can be transfected using lipid-based transfection reagents, such as FreeStyle MAX reagent

• Gibco FreeStyle CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest
• Gibco chemically defined media contain no proteins, hydrolysates, or components of unknown composition

• Protein production using Gibco FreeStyle CHO Expression Medium can be scaled up in spinner flasks or bioreactors
• The appropriate spinner or impeller speed and seeding density should be optimized for each system
• Depending on the impeller design and speed, it may be necessary to supplement Gibco FreeStyle CHO Expression Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture