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GE Healthcare Protein A and Protein G Mag Sepharose™

$207.28 - $810.96

Specifications

Matrix Paramagnetic spherical, highly cross linked agarose particles
Particle Size 37 to 100μm
Storage Requirements 4° to 8°C
View More Specs
Products
Catalog Number Mfr. No. Bead Type Binding Capacity Quantity Ligand Price Quantity    

45-002-161

 
ge healthcare
28-9440-06
Protein A Mag Sepharose 8 to 17mg Human IgG/mL 500μL Native Protein A Each for $207.28

45-002-160

 
ge healthcare
28-9440-08
Protein G Mag Sepharose 13 to 22mg Human IgG/mL 500μL Protein G Each for $207.28

45-002-173

 
ge healthcare
28-9513-79
Protein G Mag Sepharose 13 to 22mg Human IgG/mL 4 x 500μL Protein G Pack of 4 Each for $810.96

45-002-174

 
ge healthcare
28-9513-78
Protein A Mag Sepharose 8 to 17mg Human IgG/mL 4 x 500μL Native Protein A Pack of 4 Each for $810.96
Description & Specifications

Specifications

Matrix Paramagnetic spherical, highly cross linked agarose particles
Particle Size 37 to 100μm
Storage Requirements 4° to 8°C

Protein A and Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A or native protein G as ligand. Protein A and Protein G have high affinities for the Fc region of IgG antibodies and offer differing affinity characteristics across a broad range of mammalian IgG species.

There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for for downstream applications such as electrophoresis and MS.

Protein A and Protein G Mag Sepharose combine well-proven enrichment methods with the magnetic beads platform, which has ideal properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

  • Visible and dense Sepharose based magnetic beads with protein A or protein G ligand, easy to spot and collect bound target protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
  • Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed

Matrix: Paramagnetic, spherical, highly cross-linked agarose particles
Ligand: Native protein A; Native protein G
Binding capacity Protein A: 8-17mg human IgG/mL gel
Binding capacity Protein G: 13-22mg human IgG/mL gel
Particle size: 37-100μm
Working temperature: Room temperature
Storage solution: 20% ethanol
Storage temperature: 4°C to 8°C