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GE Healthcare Protein A and Protein G Mag Sepharose™

$214.72 - $840.95

Specifications

Matrix Paramagnetic spherical, highly cross linked agarose particles
Particle Size 37 to 100μm
Storage Requirements 4° to 8°C
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Products 4
Catalog Number Mfr. No. Bead Type Binding Capacity Ligand Quantity Price Quantity  
Catalog Number Mfr. No. Bead Type Binding Capacity Ligand Quantity Price Quantity  
45-002-161 View Documents GE Healthcare
28944006
Protein A Mag Sepharose 8 to 17mg Human IgG/mL Native Protein A 500μL Each for $214.72
 
45-002-160 View Documents GE Healthcare
28944008
Protein G Mag Sepharose 13 to 22mg Human IgG/mL Protein G 500μL Each for $214.72
 
45-002-173 View Documents GE Healthcare
28951379
Protein G Mag Sepharose 13 to 22mg Human IgG/mL Protein G 4 x 500μL Pack of 4 Each for $840.51
 
45-002-174 View Documents GE Healthcare
28951378
Protein A Mag Sepharose 8 to 17mg Human IgG/mL Native Protein A 4 x 500μL Pack of 4 Each for $840.95
 
Description

Description

Protein A and Protein G Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A or native protein G as ligand. Protein A and Protein G have high affinities for the Fc region of IgG antibodies and offer differing affinity characteristics across a broad range of mammalian IgG species.

There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for for downstream applications such as electrophoresis and MS.

Protein A and Protein G Mag Sepharose combine well-proven enrichment methods with the magnetic beads platform, which has ideal properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

  • Visible and dense Sepharose based magnetic beads with protein A or protein G ligand, easy to spot and collect bound target protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
  • Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed

Matrix: Paramagnetic, spherical, highly cross-linked agarose particles
Ligand: Native protein A; Native protein G
Binding capacity Protein A: 8-17mg human IgG/mL gel
Binding capacity Protein G: 13-22mg human IgG/mL gel
Particle size: 37-100μm
Working temperature: Room temperature
Storage solution: 20% ethanol
Storage temperature: 4°C to 8°C

Specifications

Specifications

Paramagnetic spherical, highly cross linked agarose particles
37 to 100μm
4° to 8°C