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Thermo Scientific™ RNase H (5 U/μL)
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.
$419.91 - $1831.91
Specifications
| Concentration | 5 U/μL |
|---|---|
| Enzyme | RNase |
| Compatible Buffer | 10X Reaction Buffer |
| Product Type | RNase H |
Description
 Ribonuclease H (Rnase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.
Applications- Removal of mRNA prior to synthesis of second strand cDNA
- RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
- Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
- Site-specific cleavage of RNA
- Studies of in vitro polyadenylation reaction products
Specifications
| 5 U/μL | |
| RNase | |
| 10X Reaction Buffer | |
| RNase H |
For Research Use Only. Not for use in diagnostic procedures.