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Thermo Scientific™ RNase H

Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand in RNA-DNA hybrids.

$229.95 - $919.80

Specifications

Concentration 5U/µL
Inhibitors metal chelators, SH-blocking reagents
Molecular Weight 18.4kDa monomer
Source E.coli MRE-600 cells
View More Specs
Products
Catalog Number Mfr. No. Units Price Quantity    

FEREN0201

 
thermo scientific™
EN0201
100U Each for $229.95

FEREN0202

 
thermo scientific™
EN0202
500U Each for $919.80
Description & Specifications

Specifications

Concentration 5U/µL
Inhibitors metal chelators, SH-blocking reagents
Molecular Weight 18.4kDa monomer
Source E.coli MRE-600 cells

 Ribonuclease H (Rnase H) specifically degrades the RNA strand in RNA-DNA hybrids. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.

Applications

  • Removal of mRNA prior to synthesis of second strand cDNA
  • RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis
  • Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT)
  • Site-specific cleavage of RNA
  • Studies of in vitro polyadenylation reaction products