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  5. Thermo Scientific™ MunI (MfeI) (10 U/μL)

Thermo Scientific™ MunI (MfeI) (10 U/μL)

Numéro de catalogue. ferer0751
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300 units
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FERER0751 300 units
FERER0752 1,500 units
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Numéro de catalogue. FERER0751 Fournisseur Thermo Scientific™ Code fournisseur ER0751
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Description

The MunI (MfeI) restriction enzyme recognizes C^AATTG sites and cuts best at 37°C in G buffer (Isoschizomers: MfeI).

Lambda DNA digested with MunI (MfeI), 0.7% agarose, 8 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C▵A A T T G...3'

3'...G T T A A▵C...5'

Conditions for 100% Activity:

  • 1X Buffer G: 10mM Tris-HCl (pH7.5 at 37°C), 10mM MgCl2, 50mM NaCl and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • MunI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: may overlap —effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5U of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16 hours

Compatible Ends:

  • EcoRI, TasI, XapI

Spécifications

Concentration 10 U/μL
Methylation Sensitivity Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Enzyme MunI (MfeI)
Compatible Buffer 10X Buffer G
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantity 300 units
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.