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  5. Thermo Scientific™ HpaII (10 U/μL)

Thermo Scientific™ HpaII (10 U/μL)

Numéro de catalogue. p-4521793
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1,000 units
5,000 units
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Numéro de catalogue. Quantity
FERER0511 1,000 units
FERER0512 5,000 units
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Numéro de catalogue. FERER0511 Fournisseur Thermo Scientific™ Code fournisseur ER0511
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Description

The HpaII restriction enzyme recognizes C^CGG sites and cuts best at 37°C in Tango buffer.

Lambda DNA digested with HpaII, 1.4% agarose, 328 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C▵C G G...3'

3'...G G C▵C...5' Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • HpaII is supplied in: 10mM Tris-HCl (pH7.4 at 25°C), 100mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: never overlaps —no effect
  • CpG: completely overlaps —blocked
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Compatible Ends:

  • Bsp119I, Bsu15I, Hin1I, MaeII, NarI, Psp1406I, SsiI, TaqI, XmiI

Spécifications

Concentration 10 U/μL
Methylation Sensitivity CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Enzyme Hpa II
Compatible Buffer 10X Buffer Tango
Sensitive to Heat Inactivation Yes
Optimal Reaction Temperature 37°C
Type IIS RE No
Quantity 1,000 units
Product Type Restriction Enzyme
Research Category Traditional Cloning

For Research Use Only. Not for use in diagnostic procedures.