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Thermo Scientific™ GeneJET™ Plasmid Maxiprep Kit

Perform large-scale isolation of high-quality plasmid DNA from recombinant E. coli cultures with this kit.

$219.45 - $487.20

Specifications

For Use With (Application) Transfection of robust cell lines, FastDigest®, restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,in vitro transcription.
Includes Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Maxi Purification Columns pre-assembled with collection tubes (50mL) and Collection tubes (50mL).
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Products
Catalog Number Mfr. No. No. of Reactions Price Quantity    

FERK0491

 
thermo scientific™
K0491
10 Preps Each for $219.45

FERK0492

 
thermo scientific™
K0492
25 Preps Each for $487.20
Description & Specifications

Specifications

For Use With (Application) Transfection of robust cell lines, FastDigest®, restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,in vitro transcription.
Includes Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Maxi Purification Columns pre-assembled with collection tubes (50mL) and Collection tubes (50mL).

GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

  • Efficient - high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
  • High concentration of DNA purified - > 400 ng/µL
  • Convenient - spin column format, centrifuge or vacuum protocols
  • Fast - procedure takes 60 minutes, considerably faster than using gravity flow columns