GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.
- Efficient - high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture
- High concentration of DNA purified - > 400 ng/µL
- Convenient - spin column format, centrifuge or vacuum protocols
- Fast - procedure takes 60 minutes, considerably faster than using gravity flow columns
|Transfection of robust cell lines, FastDigest®, restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,in vitro transcription.|
|Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Maxi Purification Columns pre-assembled with collection tubes (50mL) and Collection tubes (50mL).|