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  5. Thermo Scientific™ GeneJET Plasmid Maxiprep Kit

Thermo Scientific™ GeneJET Plasmid Maxiprep Kit

Numéro de catalogue. FERK0492
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No. of Reactions:
10 Preps
25 Preps
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Numéro de catalogue. No. of Reactions
FERK0492 25 Preps
FERK0491 10 Preps
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Numéro de catalogue. FERK0492 Fournisseur Thermo Scientific™ Code fournisseur K0492
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Description

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column.

Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers greater than 400 ng/μL of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

Highlights

  • Efficient—high yields up to 750 μg of high quality plasmid DNA from 250 mL of bacterial culture
  • High concentration of DNA purified— >400 ng/μL
  • Convenient—spin column format, centrifuge or vacuum protocols
  • Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns

Applications

Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:

  • Transfection of robust cell lines
  • FastDigest restriction enzyme digestion
  • Automated fluorescent sequencing
  • PCR
  • Cloning
  • Transformation
  • In vitro transcription

Spécifications

Concentration 400 ng/μL
Column Type Spin Column
Format Spin Column
Isolation Technology Silica Column
Scale Maxi
Sample Type Bacteria
For Use With (Application) Real-Time Quantitative PCR (qPCR), Transfection, Cloning, Sequencing, In Vitro Transcription, Transformation, Nucleic Acid Labeling, PCR
High-throughput Compatibility Not High-throughput Compatible (Manual)
No. of Reactions 25 Preps
Prep Scale > 200 μg (Large-Scale) Plasmid DNA
Product Line GeneJET
Product Type Plasmid MaxiPrep Kit
Quantity 25 preps
Target Plasmid DNA
Test Time 60 min.
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For Research Use Only. Not for use in diagnostic procedures.