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Thermo Scientific™ CpG Methyltransferase (M.SssI)

Get fast reaction times without compromising reaction efficiency with Thermo Scientific™ CpG Methyltransferase (M.SssI), one of the basic tools used in epigenetic studies.

Supplier:  Thermo Scientific™ EM0821

Numéro de catalogue. FEREM0821


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Description

Description

The enzyme methylates the C5 position on the base moiety of all cytosine nucleotides contained in unmethylated or hemimethylated double stranded DNA in a 5'-CpG-3' context.

M.SssI is specifically formulated for fast reaction times without compromising the reaction efficiency. The enzyme completes modification of all CpGs in 15 minutes at 37°C. In addition, the enzyme has been specifically validated for use with genomic DNA -the primary substrate in epigenetic studies.

M.SssI is supplied with an optimized 10X reaction buffer and 50X S-adenosylmethionine (SAM) as a cofactor.

Highlights

  • High efficiency -complete in vitro methylation of all CpGs in non-methylated and hemi-methylated DNA
  • Fast -reaction is completed in 15 minutes at 37°C
  • Stable -at -20°C for 2 years
Spécifications

Spécifications

Methyltransferase
10X Reaction Buffer
25 μL
CpG Methyltransferase
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For Research Use Only. Not for use in diagnostic procedures.