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Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin, No Weigh Format

Catalog No. pi21331
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100 mg
10 x 1 mg
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PI21331 100 mg
PIA39258 10 x 1 mg
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Catalog No. PI21331 Supplier Thermo Scientific™ Supplier No. 21331
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Description

Thermo Scientific™ EZ-Link™ Sulfo-NHS-SS-Biotin No-Weigh™ Format is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.

Thermo Scientific™ EZ-Link Sulfo-NHS-SS-Biotin enables simple and efficient biotin labeling of antibodies, proteins and any other primary amine-containing biomolecules in solution.

Highlights:

  • Cleavable analog of Sulfo-NHS-LC-Biotin
  • Can remove biotin label by cleaving spacer disulfide bond with reducing agents, such as DTT
  • Ideal for affinity purification experiments that require efficient elution from immobilized avidin supports (cleaving disulfide bond releases proteins)
  • Long spacer arm reduces steric hindrance for binding interactions

Specifications

Description EZ-Link Sulfo-NHS-SS-Biotin
Product Type Sulfo-NHS-SS-Biotin
Quantity 100 mg
Content And Storage Store at 4°C.
Product Line EZ-Link
Cell Permeability Cell-Impermeant
Chemical Reactivity Amine
Reactive Moiety Active Ester, Succinimidyl Ester, Sulfo-NHS Ester
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Label or Dye Biotin
Spacer Mid-length, Cleavable
Label Type Biotin & Analogs
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Frequently Asked Questions (FAQs)

Is there a cleavable analog of Sulfo-NHS-LC-Biotin?

Yes, we offer Sulfo-NHS-SS-Biotin (Cat. Nos. 21331, 21328). It has a disulfide bond in the spacer arm that can be cleaved with DTT or other reducing agents, thereby releasing the biotin tag from the previously labeled protein. This product is also available in kit format (Cat. Nos. 21945, 21445).
Can you provide the shelf-life for EZ-Link Sulfo-NHS-SS-Biotin?

EZ-Link Sulfo-NHS-SS-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
What product should I choose if I want to reversibly biotinylate a protein with a water soluble, amine-reactive biotin?

We recommend using EZ-Link Sulfo-NHS-SS Biotin (Cat. No. 21331 or 21338).
Why would I chose a Sulfo-NHS Biotin over its non-sulfonated counterpart?

The sodium sulfonate group lends water solubility to the reagent.
Note: The actual biotin moiety that is added is identical between NHS and Sulfo-NHS biotins that vary only in the addition of the sodium sulfonate on the leaving group. Researchers preferring a more water soluble biotin be added to their molecule should consider a PEGylated version of biotin.

For Research Use Only. Not for use in diagnostic procedures.