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  6. Thermo Scientific™ EZ-Link™ NHS-PEG12-Biotin, No-Weigh™ Format

Thermo Scientific™ EZ-Link™ NHS-PEG12-Biotin, No-Weigh™ Format

Catalog No. PIA35389
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25 mg
10 x 1 mg
500 mg
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Catalog No. Quantity
PIA35389 10 x 1 mg
PI21312 25 mg
PI21313 500 mg
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Catalog No. PIA35389 Supplier Thermo Scientific™ Supplier No. A35389
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Description

Biotinylate protein amines and maximize solubility of proteins with this very long (56.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.

Thermo Scientific™ EZ-Link NHS-PEG12-Biotin is very long, pegylated biotinylation reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.

Thermo Scientific No-Weigh products are specialty reagents provided in a pre-aliquoted format. The pre-weighed packaging helps prevent the loss of reagent reactivity and contamination over time by minimizing the repetitive opening and closing of the vial. The format enables use of a fresh vial of reagent each time, eliminating the hassle of weighing small amounts of reagents and reducing concerns over reagent stability.

  • Protein labeling: Biotinylate antibodies or other proteins for detection or purification using streptavidin probes or resins
  • Amine-reactive: Reacts with primary amines (-NH2), such as side-chain of lysines (K) or amino-termini of polypeptides
  • PEGylated: Spacer arm contains hydrophilic, 12-unit, polyethylene glycol (PEG) group
  • Enhances solubility: PEGylation imparts water solubility to biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
  • Irreversible: Forms permanent amide bonds; spacer arm cannot be cleaved
  • Very long reach: Spacer arm (total length added to target) is 56A0; this reduces steric hindrance when binding to avidin molecules

Specifications

Quantity 10 x 1 mg
Content And Storage Store desiccated at -5 to -30°C.
Shipping Condition Room Temperature
Product Line EZ-Link, No-Weigh
Solubility Water
Format Solid
Label Type Biotin & Analogs

Frequently Asked Questions (FAQs)

Can you provide the shelf-life for EZ-Link NHS-PEG12-Biotin?

EZ-Link NHS-PEG12-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (-20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
What is the advantage of using a PEGylated NHS biotinylation reagent?

The hydrophilic polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule. For example, antibodies labeled with NHS-PEG4- Biotin exhibit less aggregation when stored in solution as compared to antibodies labeled with reagents having only hydrocarbon spacers. Specific labeling of cell surface proteins is another useful application for these water-soluble and membrane-impermeable reagents.

For Research Use Only. Not for use in diagnostic procedures.