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Thermo Scientific™ EZ-Link™ Iodoacetyl-LC-Biotin

Catalog No. PI21333
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50 mg
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Catalog No. Quantity
PI21333 50 mg
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Catalog No. PI21333 Supplier Thermo Scientific™ Supplier No. 21333
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Description

Biotinylate reduced cysteines (-SH groups) at high pH via irreversible thioether bonds with this haloacetyl-activated, sulfhydryl-reactive biotinylation reagent.

Thermo Scientific™ EZ-Link Iodoacetyl-LC-Biotin is a mid-length, haloacetyl-activated, sulfhydryl-reactive biotinylation reagent that forms stable, irreversible thioether bonds at alkaline pH.

Highlights:

  • Reacts with sulfhydryl groups at pH 7.5-8.5
  • Must be dissolved in DMSO or DMF before use
  • Forms a stable thioether bond

Specifications

Product Type Iodoacetyl-LC-Biotin
Quantity 50 mg
Content And Storage Store desiccated at 4°C. Shipped at ambient temperature.
Product Line EZ-Link
Cell Permeability Cell-Permeant
Chemical Reactivity Thiol
Reactive Moiety Haloacetyl
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Label or Dye Biotin
Spacer Long
Label Type Biotin & Analogs
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Frequently Asked Questions (FAQs)

What is the advantage of using EZ-Link HPDP-Biotin over maleimide- or iodoacetyl-containing biotinylation reagents?

Both maleimide- and iodoacetyl-containing biotinylation reagents react with sulfhydryl (-SH) groups to form stable thioether bonds that are not cleavable. EZ-Link HPDP-Biotin on the other hand is a pyridyldithiol-biotin compound that reacts with -SH groups in near-neutral buffers to form reversible disulfide bonds. Because the disulfide group can be cleaved using DTT or other reducing agents, HPDP-Biotin is useful for labeling and affinity-purification applications that require recovery of the original, unmodified molecule.

For Research Use Only. Not for use in diagnostic procedures.