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Thermo Scientific™ EZ-Link™ BMCC-Biotin

Catalog No. PI21900
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50 mg
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Catalog No. Quantity
PI21900 50 mg
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Catalog No. PI21900 Supplier Thermo Scientific™ Supplier No. LSG21900
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Description

Biotinylate reduced cysteines (-SH groups) with a maleimide-activated, sulfhydryl-reactive biotinylation reagent containing a stabilizing cyclohexane group in the spacer arm.

Thermo Scientific™ EZ-Link BMCC-Biotin is a maleimide-activated, sulfhydryl-reactive biotinylation reagent with an extended spacer arm that contains a stabilizing cyclohexane group.

Highlights:

  • Biotinylates at pH 6.5-7.5
  • Reaction avoids potential modification of tyrosine groups that can inactivate biological activity
  • Longer spacer arm than iodoacetyl biotin
  • Must be dissolved in DMSO or DMF before use

Specifications

Product Type BMCC-Biotin
Quantity 50 mg
Content And Storage Store at 4°C.
Product Line EZ-Link
Cell Permeability Cell-Permeant
Chemical Reactivity Thiol
Reactive Moiety Maleimide
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Label or Dye Biotin
Spacer Extra-long
Label Type Biotin & Analogs
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Frequently Asked Questions (FAQs)

What is the advantage of using EZ-Link HPDP-Biotin over maleimide- or iodoacetyl-containing biotinylation reagents?

Both maleimide- and iodoacetyl-containing biotinylation reagents react with sulfhydryl (-SH) groups to form stable thioether bonds that are not cleavable. EZ-Link HPDP-Biotin on the other hand is a pyridyldithiol-biotin compound that reacts with -SH groups in near-neutral buffers to form reversible disulfide bonds. Because the disulfide group can be cleaved using DTT or other reducing agents, HPDP-Biotin is useful for labeling and affinity-purification applications that require recovery of the original, unmodified molecule.

For Research Use Only. Not for use in diagnostic procedures.