Thermo Scientific™ EZ-Link™ Amine-PEG11-Biotin

Description

Thermo Scientific™ EZ-Link Amine-PEG11-Biotin is a biotinylation reagent containing a long 11-unit polyethylene glycol (PEG) spacer arm and a terminal primary amine group for conjugation and protein labeling with EDC or other crosslinkers.

EZ-Link Amine-PEGn-Biotin reagents are water-soluble, polyethylene glycol (PEG)-containing reagents with terminal primary amines (NH₂). The PEG spacer arm is hydrophilic and confers greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The amine group of these reagents can be reacted with carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the NH₂ group of the amine-biotin, forming an amide bond.

Highlights:

• Biotin – label macromolecules for detection or affinity purification with avidin and streptavidin

• Amine-activated – primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers

• Polyethylene glycol – PEG groups are flexible, non-immunogenic, hydrophilic, and improve the solubility of labeled antibodies

• Spacer arm – the reach between the point of attachment and the biotin affinity tag is more than 50 angstroms, minimizing steric hindrance for the biotin binding-function

Specifications:

• Chemical name (simplified): biotinyl-undecaoxapentatriacontanediamine
• Chemical formula: C₃₄H₆₆N₄O₁₃S
• Molecular weight: 770.97
• Spacer arm: 53.2 angstroms
• Form: white to off-white solid
• Solubility: at least 25mg/mL in water or buffer