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Thermo Scientific™ Exonuclease I (Exo I)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
20% (w/v) PEG 8000 (5)
E.coli cells with a cloned E.coli sbcB gene
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The purchase of this product allows the purchaser to use it for preparing amplified DNA fragments under a license from GE Healthcare of U.S. Patent Nos. 5,741,676 and 5,756,285 and other foreign patents.
Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.
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Inhibitors 20% (w/v) PEG 8000 (5)
Molecular Weight 54.5kDa monomer
Source E.coli cells with a cloned E.coli sbcB gene