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  5. Thermo Scientific™ Exonuclease I (20 U/μL)

Thermo Scientific™ Exonuclease I (20 U/μL)

Catalog No. FEREN0581
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20,000 units
4,000 units
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FEREN0581 4,000 units
FEREN0582 20,000 units
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Catalog No. FEREN0581 Supplier Thermo Scientific™ Supplier No. EN0581
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Description

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

  • Active in PCR buffers

Applications

  • Primer removal from PCR mixtures:
    • prior to PCR product sequencing
      • li>for one-tube "megaprimer" PCR mutagenesis
  • Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
  • Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

Specifications

Concentration 20 U/μL
Enzyme Exonuclease
Quantity 4,000 units
Product Type Exonuclease I

For Research Use Only. Not for use in diagnostic procedures.