missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Modifying Enzymes
  5. Thermo Scientific™ Exonuclease I (20 U/μL)

Thermo Scientific™ Exonuclease I (20 U/μL)

Catalog No. FEREN0582
Change view
Click to view available options
Quantity:
20,000 units
4,000 units
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
FEREN0582 20,000 units
FEREN0581 4,000 units
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. FEREN0582 Supplier Thermo Scientific™ Supplier No. EN0582
Only null left

Description

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Exonuclease I (ExoI) degrades single-stranded DNA in a 3'≥5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.

It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.

Highlights

  • Active in PCR buffers

Applications

  • Primer removal from PCR mixtures:
    • prior to PCR product sequencing
      • li>for one-tube "megaprimer" PCR mutagenesis
  • Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
  • Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus

Note

The enzyme is not suitable for removing 3'-overhangs of dsDNA.

Specifications

Concentration 20 U/μL
Enzyme Exonuclease
Quantity 20,000 units
Product Type Exonuclease I

For Research Use Only. Not for use in diagnostic procedures.