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Invitrogen™ Mouse Eotaxin-2 (CCL24) ELISA Kit 
ELISA
Supplier: Invitrogen™ EMCCL24
Description
The Mouse Eotaxin-2 (CCL24) ELISA quantitates Ms Eotaxin-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Eotaxin-2. Principle of the method The Mouse Eotaxin-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
Eotaxin 2 belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.Specifications
| Q9JKC0 | |
| 4 pg/mL | |
| Solid-phase sandwich ELISA | |
| ELISA | |
| Plasma, Serum, Supernatant | |
| 96 assays | |
| ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis | |
| 56221 | |
| <12% | |
| Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers | |
| 96 Tests | |
| Inflammation | |
| 2°C to 8°C | |
| Eotaxin 2 | |
| 4 hrs 45 mins |
| 3.28 to 800 pg/mL | |
| 4 pg/mL to 800 pg/mL | |
| HRP | |
| Cytokines & Receptors | |
| This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. | |
| Mouse | |
| Colorimetric Microplate Reader | |
| CKb-6,MPIF-2,Scya24 | |
| <10 | |
| HRP | |
| RUO | |
| Plasma 50 μL, Serum 50 μL, Supernatant 100 μL | |
| CKb-6, MPIF-2, Scya24 | |
| 1 hrs 20 mins |