MilliporeSigma™ pET-48b(+) DNA Vector

The pET-48b(+) vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx·Tag™ thioredoxin protein that is cleavable with the human rhinovirus (HRV) 3C protease. Cloning sites are available for producing fusion proteins also containing a cleavable N- terminal His·Tag™ sequence for detection and purification. The plasmid contains a strong T7lac promoter, optimized RBS, the coding sequence for the rhinovirus 3C protease cleavage site (LeuGluValLeuPheGlnGlyPro) for N-terminal fusion tag removal and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S·Tag™ coding sequence is compatible with purification, detection, and quantification.