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Thermo Scientific™ E. coli DNA Ligase
Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.
Supplier: Thermo Scientific™ EL00361
Description
Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. The enzyme is not active on blunt-ended DNA.
Features
- Activity in wide temperature range (4°C to 37°C)
- High specificity to cohesive DNA ends
Applications
- Nick ligation in double-stranded DNA
- cDNA synthesis
- Cloning
Specifications
| 8 U/μL | |
| 8 U/μL E.Coli DNA Ligase, 50 μL Store at -20°C. |
|
| Yes | |
| NAD-dependent DNA Ligase | |
| 65°C |
| Nick ligation in double-stranded DNA, cDNA synthesis, Cloning, DNA ligation | |
| DNA Ligase | |
| 400 U | |
| Traditional Cloning | |
| Liquid |
For Research Use Only. Not for use in diagnostic procedures.