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Thermo Scientific™ E. coli DNA Ligase

Catalog No. FEREL00361
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Quantity:
400 U
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Catalog No. Quantity
FEREL00361 400 U
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Catalog No. FEREL00361 Supplier Thermo Scientific™ Supplier No. EL00361
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Description

Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.

Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. The enzyme is not active on blunt-ended DNA.

Features

  • Activity in wide temperature range (4°C to 37°C)
  • High specificity to cohesive DNA ends

Applications

  • Nick ligation in double-stranded DNA
  • cDNA synthesis
  • Cloning

Specifications

Concentration 8 U/μL
For Use With (Application) Nick ligation in double-stranded DNA, cDNA synthesis, Cloning, DNA ligation
Content And Storage 8 U/μL E.Coli DNA Ligase, 50 μL

Store at -20°C.
Enzyme DNA Ligase
Sensitive to Heat Inactivation Yes
Quantity 400 U
Product Type NAD-dependent DNA Ligase
Research Category Traditional Cloning
Inactivation Temperature 65°C
Form Liquid

For Research Use Only. Not for use in diagnostic procedures.